Anticorps Polyclonal de lapin anti-KIF3A

• Phare
• Validé par KD/KO

KIF3A Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 13930-1-AP

Synonymes

KIF3, KIF3A, kinesin family member 3A, Kinesin like protein KIF3A

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Galerie de données de validation

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  WB analysis of mouse brain using 13930-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13930-1-AP (KIF3A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 13930-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13930-1-AP (KIF3A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 13930-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13930-1-AP (KIF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13930-1-AP

  IP Result of anti-KIF3A (IP:13930-1-AP, 4ug; Detection:13930-1-AP 1:500) with mouse brain tissue lysate 8000ug.

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  IP experiment using 13930-1-AP

  WB and IP result of the antibdoy anti-KIF3A (13930-1-AP) in mouse cell from Corbit, Kevin.

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  IHC staining of human gliomas using 13930-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13930-1-AP (KIF3A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human gliomas using 13930-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13930-1-AP (KIF3A antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu de cervelet de souris, tissu de cervelet humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IF: See 4 publications below

Informations sur le produit

13930-1-AP cible KIF3A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF3A Protéine recombinante Ag4954
• Nom complet: kinesin family member 3A
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC045542
• Symbole du gène: KIF3A
• Identification du gène (NCBI): 11127
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIF3A is a subunit of Kinesin II that is essential for intraflagellar transport (IFT). It functions as the anterograde motor to deliver ciliary components to the tip where the axoneme is assembled. Disruption of KIF3A has been reported to block mammalian ciliogenesis. Recently KIF3A has been found to localize to the mother centriole and is required for centrioles organization. This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-/- lysate. (23386061)

Publications

Species	Application	Title
human	WB	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	WB	Sci Adv; An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.; Authors - Molly M Wilson
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
human	WB	Proc Natl Acad Sci U S A; EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.; Authors - Vincent J Guen ; KO Validated
mouse, human	WB,IF	EMBO J; Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.; Authors - Kodani Andrew A ; KD Validated
human	WB,IF	Cell Mol Life Sci; Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.; Authors - Delowar Hossain ; KD Validated