Anticorps Recombinant de lapin anti-KIF5A

KIF5A Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241124H1

N° de cat : 84013-4-RR

Synonymes

241124H1, D12S1889, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, MY050

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Galerie de données de validation

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  WB analysis of rat brain using 84013-4-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 84013-4-RR (KIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84013-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84013-4-RR (KIF5A antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 84013-4-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (84013-4-RR, Clone: 241124H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of K-562 using 84013-4-RR

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of SH-SY5Y using 84013-4-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84013-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84013-4-RR against Human KIF5A were performed. The affinity constant is 78.9 pM.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-12,
• Résultats positifs en FC (Intra): cellules K-562, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:125-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84013-4-RR cible KIF5A dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KIF5A Protéine recombinante Ag15518
• Nom complet: kinesin family member 5A
• Masse moléculaire calculée: 1032 aa, 117 kDa
• Poids moléculaire observé: 118-120 kDa
• Numéro d’acquisition GenBank: BC146670
• Symbole du gène: KIF5A
• Identification du gène (NCBI): 3798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

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