Anticorps Polyclonal de lapin anti-KIFAP3

• Phare
• Validé par KD/KO

KIFAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 12700-1-AP

Synonymes

Kinesin-associated protein 3, kinesin associated protein 3, KIF3AP, KAP-3, KAP3

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Galerie de données de validation

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  WB analysis of human brain using 12700-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12700-1-AP

  WB result of KIFAP3 antibody (12700-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIFAP3 transfected MCF-7 cells.

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  WB analysis of mouse testis using 12700-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12700-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12700-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12700-1-AP

  IP result of anti-KIFAP3 (IP:12700-1-AP, 4ug; Detection:12700-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human ovary cancer using 12700-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12700-1-AP (KIFAP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12700-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12700-1-AP (KIFAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules MCF-7, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12700-1-AP cible KIFAP3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIFAP3 Protéine recombinante Ag3394
• Nom complet: kinesin-associated protein 3
• Masse moléculaire calculée: 792 aa, 91 kDa
• Poids moléculaire observé: 91-100 kDa
• Numéro d’acquisition GenBank: BC028679
• Symbole du gène: KIFAP3
• Identification du gène (NCBI): 22920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIFAP3, also known as KIF3AP or KAP3, is a novel KIF3A/3B-associated protein. It binds to the tail domain of KIF3A/3B and may play a role in regulating the binding of KIF3A/3B to cargoes. Recently it has been reported that mutation within the KIFAP3 gene is associated with decreased KIFAP3 expression and increased survival in sporadic ALS, which makes it a potential target for ALS therapy. KIFAP3 has also been identified as the target of mir-130a.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; A functional assay for microRNA target identification and validation.; Authors - G?ken Joop J
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes

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