Anticorps Polyclonal de lapin anti-KIFC1

• Phare
• Validé par KD/KO

KIFC1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Peptide array, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 20790-1-AP

Synonymes

HSET, KIFC1, kinesin family member C1, Kinesin like protein 2, Kinesin like protein KIFC1, Kinesin related protein HSET, KNSL2

---

Galerie de données de validation

• 

  WB analysis of rat testis using 20790-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20790-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20790-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20790-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20790-1-AP (KIFC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20790-1-AP (KIFC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20790-1-AP (KIFC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20790-1-AP (KIFC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20790-1-AP

  Immunofluorescent analysis of Hela cells, using KIFC1 antibody 20790-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 20790-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIFC1 antibody 20790-1-AP at 1:25 dilution and FITC- labeled donkey anti-Rabbit IgG (green).

---

Applications testées

• Résultats positifs en WB: tissu testiculaire de rat, cellules HeLa, cellules NIH/3T3, tissu testiculaire de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

20790-1-AP cible KIFC1 dans les applications de WB, Peptide array, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIFC1 Protéine recombinante Ag14492
• Nom complet: kinesin family member C1
• Masse moléculaire calculée: 673 aa, 74 kDa
• Poids moléculaire observé: 55-70 kDa
• Numéro d’acquisition GenBank: BC000712
• Symbole du gène: KIFC1
• Identification du gène (NCBI): 3833
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport. This antibody was raised against the C-terminal region of human KIFC1 protein, and recognizes 55-70 kDa of endogenous KIFC1 protein in multiple tissue lysates. (12826589, 15236353, 23543461)

Publications

Species	Application	Title
mouse	WB,IF,Peptide array	Science; Mechanism of spindle pole organization and instability in human oocytes.; Authors - Chun So ; KD Validated
human	WB	Dev Cell; Direct Microtubule-Binding by Myosin-10 Orients Centrosomes toward Retraction Fibers and Subcortical Actin Clouds.; Authors - Mijung Kwon
human	WB	Cell Mol Life Sci; Proteomic interaction profiling reveals KIFC1 as a factor involved in early targeting of F508del-CFTR to degradation.; Authors - Sara Canato ; KD Validated
rat	WB	J Neurosci; KIFC1 regulates the trajectory of neuronal migration.; Authors - Hemalatha Muralidharan ; KD Validated
human	IF	FASEB J; Export of membrane proteins from the Golgi complex to the primary cilium requires the kinesin motor, KIFC1.; Authors - Si-Hyung Lee
human	IHC	Front Mol Biosci; Prognostic Value and Immunological Role of KIFC1 in Hepatocellular Carcinoma.; Authors - Dan Li