Anticorps Polyclonal de lapin anti-KIN17

KIN17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 12313-1-AP

Synonymes

KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA

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Galerie de données de validation

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  WB analysis of HeLa using 12313-1-AP

  HeLa cells, mouse testis tissue, and rat testis tissue were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12313-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12313-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12313-1-AP

  IP result of anti-KIN17 (IP:12313-1-AP, 4ug; Detection:12313-1-AP 1:500) with mouse brain tissue lysate 3160 ug.

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  IHC staining of human ovary cancer using 12313-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12313-1-AP (KIN17 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12313-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIN17 antibody (12313-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cardiaque humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12313-1-AP cible KIN17 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIN17 Protéine recombinante Ag2963
• Nom complet: KIN, antigenic determinant of recA protein homolog (mouse)
• Masse moléculaire calculée: 393 aa, 45 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC017309
• Symbole du gène: KIN17
• Identification du gène (NCBI): 22944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIN17 (also known as KIN) is an evolutionarily conserved zinc finger nuclear protein. It consists of three motifs, a zinc finger, a nuclear localization signal and a Kyprides-Onzonis-Woese motif that mediates transcription elongation. KIN17 is ubiquitously expressed in human tissues and participates in DNA replication, DNA repair and cell cycle progression. Emerging evidence has indicated the importance of KIN17 in the pathogenesis and progression of tumors (PMID: 28454391). KIN17 has a calculated molecular wight of 45 kDa, this antibody is raised against human KIN17 and detects a band of about 40 kDa.

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