Anticorps Polyclonal de lapin anti-KIR2DS4/CD158i

KIR2DS4/CD158i Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 21152-1-AP

Synonymes

KIR2DS4, CD158 antigen-like family member I, CD158I, Killer cell immunoglobulin-like receptor 2DS4, KIR1D

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Galerie de données de validation

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  WB analysis of HL-60 using 21152-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21152-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21152-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 21152-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIR2DS4/CD158i antibody 21152-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21152-1-AP cible KIR2DS4/CD158i dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIR2DS4/CD158i Protéine recombinante Ag15711
• Nom complet: killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4
• Masse moléculaire calculée: 304 aa, 34 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC096694
• Symbole du gène: KIR2DS4
• Identification du gène (NCBI): 3809
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIR2DS4 (also known as CD158i) is a member of the killer cell immunoglobulin-like receptor (KIR) family. KIRs are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. KIRs are mainly involved in inhibiting NK killing (inhibitory KIRs) via interaction with MHC class I molecules. KIR2DS4 has been reported to be an activating KIR and recognize the HLA-Cw4 protein.

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