Anticorps Polyclonal de lapin anti-KISS1

• Phare
• Validé par KD/KO

KISS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 18375-1-AP

Synonymes

KiSS 1, KiSS 1 metastasis suppressor, KISS1, Kisspeptin 1, Metastasis suppressor KiSS 1, metastin

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 18375-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18375-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18375-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18375-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 18375-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 18375-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18375-1-AP (KISS1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of NIH/3T3 using 18375-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 18375-1-AP (KISS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu cérébral de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Informations sur le produit

18375-1-AP cible KISS1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KISS1 Protéine recombinante Ag13240
• Nom complet: KiSS-1 metastasis-suppressor
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 16-20 kDa
• Numéro d’acquisition GenBank: BC022819
• Symbole du gène: KISS1
• Identification du gène (NCBI): 3814
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).

Publications

Species	Application	Title
human	WB,IHC	Int J Cancer; Long noncoding RNA TC0101441 induces epithelial-mesenchymal transition in epithelial ovarian cancer metastasis by downregulating KiSS1.; Authors - Jun-Jun Qiu ; KD Validated
human	IHC	Histochem Cell Biol; Functional analysis of miR-767-5p during the progression of hepatocellular carcinoma and the clinical relevance of its dysregulation.; Authors - Lei Zhang
human	IHC	Oncol Lett; Analysis of the association of the expression of KiSS-1 in colorectal cancer tissues with the pathology and prognosis.; Authors - Xinkai Huo
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristina (Verified Customer) (09-18-2020)

Works great for us- seeing an increase in modified MDA231 cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MDA-MB-231 with modifications