Anticorps Polyclonal de lapin anti-KL

KL Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(27)

N° de cat : 28100-1-AP

Synonymes

alpha klotho, klotho, α klotho, α-klotho

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Galerie de données de validation

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  WB analysis using 28100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28100-1-AP (KL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 28100-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 28100-1-AP (KL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 28100-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 28100-1-AP (KL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 28100-1-AP

  IP result of anti-KL (IP:28100-1-AP, 4ug; Detection:28100-1-AP 1:300) with mouse kidney tissue lysate 4000 ug.

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  IHC staining of human kidney using 28100-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 28100-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28100-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28100-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28100-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28100-1-AP (KL antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu rénal de rat
• Résultats positifs en IP: tissu rénal de souris,
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

28100-1-AP cible KL dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KL Protéine recombinante Ag27759
• Nom complet: klotho
• Masse moléculaire calculée: 116 kDa
• Poids moléculaire observé: 62 kDa, 130 kDa
• Numéro d’acquisition GenBank: NM_004795
• Symbole du gène: KL
• Identification du gène (NCBI): 9365
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo.; Authors - Wen-Chih Liu
		Acta Pharmacol Sin; Dihydroartemisinin suppresses renal fibrosis in mice by inhibiting DNA-methyltransferase 1 and increasing Klotho.; Authors - Wei Zhou
mouse	WB	Ecotoxicol Environ Saf; Effects of selenium-cadmium co-enriched Cardamine hupingshanensis on bone damage in mice; Authors - Lin Zhang
human	IHC	Cell Mol Life Sci; α-Klotho released from HK-2 cells inhibits osteogenic differentiation of renal interstitial fibroblasts by inactivating the Wnt-β-catenin pathway.; Authors - Zewu Zhu
rat	WB	Sci Rep; PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.; Authors - Jing Li
mouse	WB	Hum Cell; Serine protease HtrA2/Omi regulates adaptive mitochondrial reprogramming in the brain cortex after ischemia/reperfusion injury via UCP2-SIRT3-PGC1 axis.; Authors - Hao Meng

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