Anticorps Polyclonal de lapin anti-KLF10

KLF10 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, Chip, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 11881-1-AP

Synonymes

EGR alpha, EGRA, KLF10, Krueppel like factor 10, Kruppel like factor 10, TIEG, TIEG 1, TIEG1

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Galerie de données de validation

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  WB analysis of HeLa using 11881-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11881-1-AP (KLF10 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11881-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11881-1-AP (KLF10 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11881-1-AP

  IP Result of anti-KLF10 (IP:11881-1-AP, 4ug; Detection:11881-1-AP 1:300) with Jurkat cells lysate 2800ug.

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  IF Staining of HeLa using 11881-1-AP

  Immunofluorescent analysis of HeLa cells using 11881-1-AP (KLF10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

11881-1-AP cible KLF10 dans les applications de WB, IP, IHC, IF, Chip, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KLF10 Protéine recombinante Ag2482
• Nom complet: Kruppel-like factor 10
• Masse moléculaire calculée: 469 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC011538
• Symbole du gène: KLF10
• Identification du gène (NCBI): 7071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KLF10 (Krueppel-like factor 10), also known as TIEG1, is a transcription factor that plays a role in the regulation of cell growth. It binds to the consensus sequence 5'-GGTGTG-3'. KLF10 plays a role in the response to TGFbeta (through the TGFbeta/Smad signalling pathway) and estrogen. In both cases, KLF10 induces or repressed gene transcription to enhance the TGFbeta/Smad signalling pathway, as well as other pathways, to regulate cell proliferation, differentiation and apoptosis. KLF10 has been shown to have a role in skeletal disease (osteopenia/osteoporosis), hypertrophic cardiomyopathy, and breast and prostate cancer.This antibody is a rabbit polyclonal antibody raised against a full-length human KLF10 protein, and specifically recognizes the 52kd KLF10 protein.

Publications

Species	Application	Title
human	Chip	Sci Rep; Polymorphism rs7278468 is associated with Age-related cataract through decreasing transcriptional activity of the CRYAA promoter.; Authors - Xiaoyin Ma
human	IF	J Mol Cell Cardiol; Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.; Authors - Yongyun Li
human	WB,IHC	Acta Histochem; KLF10 is upregulated in osteoarthritis and inhibits chondrocyte proliferation and migration by upregulating Acvr1 and suppressing inhbb expression.; Authors - Langlang Zheng
mouse,canine	IF	Cell Death Discov; Downregulation of KLF10 contributes to the regeneration of survived renal tubular cells in cisplatin-induced acute kidney injury via ZBTB7A-KLF10-PTEN axis; Authors - Yang Zhang
mouse,human	WB	Cell Signal; Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis; Authors - Xiaoyan Chen