Anticorps Polyclonal de lapin anti-KLF5

• Phare
• Validé par KD/KO

KLF5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

40

Avis (1)

N° de cat : 21017-1-AP

Synonymes

BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2

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Galerie de données de validation

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  WB analysis using 21017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21017-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21017-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 21017-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21017-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 21017-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 21017-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 21017-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21017-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21017-1-AP

  IP Result of anti-KLF5 (IP:21017-1-AP, 4ug; Detection:21017-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IHC staining of mouse embryo using 21017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21017-1-AP (KLF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21017-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21017-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 21017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21017-1-AP (KLF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21017-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21017-1-AP (KLF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Caco-2, cellules COLO 320, cellules HCT 116, cellules HeLa, cellules HT-1080, cellules PC-3, tissu de côlon de rat, tissu de côlon de souris, tissu d'intestin grêle de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu embryonnaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 31 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 4 publications below

Informations sur le produit

21017-1-AP cible KLF5 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KLF5 Protéine recombinante Ag15267
• Nom complet: Kruppel-like factor 5 (intestinal)
• Masse moléculaire calculée: 457 aa, 51 kDa
• Poids moléculaire observé: 51-55 kDa, 85-120 kDa
• Numéro d’acquisition GenBank: BC042131
• Symbole du gène: KLF5
• Identification du gène (NCBI): 688
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).

Publications

Species	Application	Title
human	WB	Cell Res; ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.; Authors - Shunrong Ji
human	WB	Cell Death Dis; DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.; Authors - Rong-Jie Fu ; KD Validated
human	WB,IHC	Aging (Albany NY); Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.; Authors - Yilong Li ; KD Validated
human	WB	Oncotarget; Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.; Authors - Xiaolong Wei ; KD Validated
mouse	WB	Eur J Pharmacol; Early growth response 1/Krüppel-like factor 5 pathway inhibitor alleviates lipopolysaccharide-induced lung injury by promoting autophagy; Authors - Kang Zou
human	WB	Front Oncol; A Weighted Gene Co-Expression Network Analysis-Derived Prognostic Model for Predicting Prognosis and Immune Infiltration in Gastric Cancer.; Authors - Qingchuan Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah-Eve (Verified Customer) (03-01-2023)

Works okay on pvdf membrane, blocking with skim milk. A lot of bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human pulmonary smooth muscle cells

KLF5 Antibody Western Blot validation (1:2000 dilution) in Human pulmonary smooth muscle cells (Cat no:21017-1-AP)