Anticorps Polyclonal de lapin anti-KLF6

KLF6 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 14716-1-AP

Synonymes

B cell derived protein 1, COPEB, CPBP, GBF, KLF6, Krueppel like factor 6, Kruppel like factor 6, PAC1, Proto oncogene BCD1, ST12, Transcription factor Zf9, ZF9

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Galerie de données de validation

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  WB analysis using 14716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14716-1-AP (KLF6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14716-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14716-1-AP (KLF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14716-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14716-1-AP (KLF6 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse colon using 14716-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14716-1-AP (KLF6 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human testis using 14716-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14716-1-AP (KLF6 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 14716-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14716-1-AP (KLF6 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HUVEC, cellules NCI-H1299, tissu de côlon de souris, tissu de thymus de souris
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

14716-1-AP cible KLF6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KLF6 Protéine recombinante Ag6440
• Nom complet: Kruppel-like factor 6
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32-42 kDa
• Numéro d’acquisition GenBank: BC000311
• Symbole du gène: KLF6
• Identification du gène (NCBI): 1316
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KLF6(krupple-like factor 6) is a zinc finger transcription factor and tumor suppressor with biological activities and transcriptional targets in growing range. It is highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. One key target gene of KLF6 in endoglin, which is a homodimeric cell membrane glycoprotein and TGF-β auxiliary receptor, owns a pro-angiogenic role in endothelial cells and is also involved in malignant progression. Also KLF6 induces apoptosis in prostate cancer cells through upregulation of ATF3. KLF6 was normal detected as a 46kDa protein, which is larger than translation product(32kDa), because of post-translational modification including both phosphorylation and glycosylation. This antibody raise against the full length of KLF6 gene of human origin, and can recognize both of KLF6, included KLF6(~32kDa), p-KLF6(~35-46kDa). Otherwise, there may be a non-specific band(50kDa) showed in detection

Publications

Species	Application	Title
human,mouse	WB,IF	Adv Sci (Weinh); Exosomal miR-181d-5p Derived from Rapamycin-Conditioned MDSC Alleviated Allograft Rejection by Targeting KLF6; Authors - Chao Wei
human	WB	Nutrients; The Growth of SGC-7901 Tumor Xenografts Was Suppressed by Chinese Bayberry Anthocyanin Extract through Upregulating KLF6 Gene Expression.; Authors - Yue Wang
mouse	WB	J Ethnopharmacol; Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose.; Authors - Jing Zhou
mouse	IF	Front Genet; Revealing Potential Spinal Cord Injury Biomarkers and Immune Cell Infiltration Characteristics in Mice.; Authors - Liang Cao
mouse	WB,IF	Front Physiol; MiR-181d-5p Targets KLF6 to Improve Ischemia/Reperfusion-Induced AKI Through Effects on Renal Function, Apoptosis, and Inflammation.; Authors - Yue Zhang
rat	WB	Bioengineered; MicroRNA-124 modulates neuroinflammation in acute methanol poisoning rats via targeting Krüppel-like factor-6.; Authors - Shu Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paulo (Verified Customer) (03-01-2019)

Used to perform ChIP-seq in ccRCC cell lines. 20ug of antibody used in 100million cells. No peaks were detected in known target genes and obtained peaks failed to present the consensus KLF6 binding site after motif analysis.

• Applications: ChIP,