Anticorps Monoclonal anti-KLF6

KLF6 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2F8B3

N° de cat : 67297-1-Ig

Synonymes

BCD1, B-cell-derived protein 1, COPEB, Core promoter element-binding protein, CPBP

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Galerie de données de validation

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  WB analysis using 67297-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67297-1-Ig (KLF6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules JAR, cellules Jurkat, cellules K-562, cellules L02, cellules NCCIT

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

67297-1-Ig cible KLF6 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KLF6 Protéine recombinante Ag6859
• Nom complet: Kruppel-like factor 6
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32-42 kDa
• Numéro d’acquisition GenBank: BC000311
• Symbole du gène: KLF6
• Identification du gène (NCBI): 1316
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KLF6(krupple-like factor 6) is a zinc finger transcription factor and tumor suppressor with biological activities and transcriptional targets in growing range. It is highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. One key target gene of KLF6 in endoglin, which is a homodimeric cell membrane glycoprotein and TGF-β auxiliary receptor, owns a pro-angiogenic role in endothelial cells and is also involved in malignant progression. Also KLF6 induces apoptosis in prostate cancer cells through upregulation of ATF3. KLF6 was normal detected as a 46kDa protein, which is larger than translation product(32kDa), because of post-translational modification including both phosphorylation and glycosylation. This antibody raise against the full length of KLF6 gene of human origin, and can recognize both of KLF6, included KLF6(~32kDa), p-KLF6(~35-46kDa). Otherwise, there may be a non-specific band(50kDa) showed in detection

Publications

Species	Application	Title
human	WB	BMC Mol Cell Biol; LncRNA LUCAT1/miR-181a-5p axis promotes proliferation and invasion of breast cancer via targeting KLF6 and KLF15.; Authors - Yun Liu
mouse,human	WB	Cell Signal; Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis; Authors - Xiaoyan Chen
mouse	WB	Life Sci; The zinc-finger transcription factor KLF6 regulates cardiac fibrosis; Authors - Nan Li

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