Anticorps Polyclonal de lapin anti-KLHL14

KLHL14 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 16693-1-AP

Synonymes

kelch like 14 (Drosophila), Kelch like protein 14, KIAA1384, KLHL14, Printor, Protein interactor of Torsin-1A

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Galerie de données de validation

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  WB analysis of PC-3 using 16693-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16693-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16693-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16693-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16693-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16693-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16693-1-AP (KLHL14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HepG2, cellules MCF-7
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16693-1-AP cible KLHL14 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KLHL14 Protéine recombinante Ag10065
• Nom complet: kelch-like 14 (Drosophila)
• Masse moléculaire calculée: 44 kDa, 71 kDa
• Poids moléculaire observé: 35 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC021267
• Symbole du gène: KLHL14
• Identification du gène (NCBI): 57565
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kelch-like protein 14 (KLHL14) is a member of the KLHL family. The kelch motif was initially discovered in Kelch. In this protein there are six copies of the motif. It has been shown for one member that it is related to Galactose Oxidase for which a structure has been solved. Two isoforms of this protein exist - 70kDa and 43kDa.