Anticorps Monoclonal anti-KLRB1/CD161

KLRB1/CD161 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

3D8F5

N° de cat : 67537-1-Ig

Synonymes

CD161, 3D8F5, CLEC5B, C-type lectin domain family 5 member B, hNKR P1A

---

Galerie de données de validation

• 

  WB analysis of human spleen using 67537-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu splénique humain,
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

67537-1-Ig cible KLRB1/CD161 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KLRB1/CD161 Protéine recombinante Ag27117
• Nom complet: killer cell lectin-like receptor subfamily B, member 1
• Masse moléculaire calculée: 225 aa, 25 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC114516
• Symbole du gène: CD161
• Identification du gène (NCBI): 3820
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).

Publications

Species	Application	Title
human	IF	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
human	IHC	Front Immunol; Comprehensive single-cell and bulk transcriptomic analyses to develop an NK cell-derived gene signature for prognostic assessment and precision medicine in breast cancer; Authors - Qianshan Hou
human	WB	J Thorac Dis; KLRB1 expression is associated with lung adenocarcinoma prognosis and immune infiltration and regulates lung adenocarcinoma cell proliferation and metastasis through the MAPK/ERK pathway; Authors - Siwei Xu
human	WB	Front Endocrinol (Lausanne); Machine learning immune-related gene based on KLRB1 model for predicting the prognosis and immune cell infiltration of breast cancer; Authors - Guo Huang
human	IHC	BMJ Open Respir Res; Single-cell RNA-sequencing uncovers the dynamic changes of tumour immune microenvironment in advanced lung adenocarcinoma; Authors - Haijiao Lu
human	IHC	Front Immunol; Single-cell sequencing analysis and multiple machine-learning models revealed the cellular crosstalk of dendritic cells and identified FABP5 and KLRB1 as novel biomarkers for psoriasis; Authors - Zhiqiang Ma

{{ptg:RelatedPrimaryAntibodies}}