Anticorps Polyclonal de lapin anti-KMO

• Phare
• Validé par KD/KO

KMO Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 10698-1-AP

Synonymes

KMO, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase

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Galerie de données de validation

• 

  WB analysis of HeLa using 10698-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10698-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10698-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 10698-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10698-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10698-1-AP (KMO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse heart using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10698-1-AP

  Immunofluorescent analysis of HepG2 cells using 10698-1-AP (KMO antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 10698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMO antibody (10698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 10698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMO antibody (10698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 10698-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 10698-1-AP (KMO antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque de souris, tissu de cancer de la prostate humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules MCF-7

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 8 publications below
• IF: See 9 publications below

Informations sur le produit

10698-1-AP cible KMO dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KMO Protéine recombinante Ag1109
• Nom complet: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Masse moléculaire calculée: 486 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC005297
• Symbole du gène: KMO
• Identification du gène (NCBI): 8564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

Publications

Species	Application	Title
mouse	IF	J Hazard Mater; Particulate matter-induced metabolic recoding of epigenetics in macrophages drives pathogenesis of chronic obstructive pulmonary disease; Authors - Myungkyung Noh
human	WB	Circ Res; Activation of NAD(P)H Oxidase by Tryptophan-Derived 3-Hydroxykynurenine Accelerates Endothelial Apoptosis and Dysfunction In Vivo.; Authors - Qiongxin Wang
human, mouse	IF	J Am Soc Nephrol; Loss of Kynurenine 3-Mono-oxygenase Causes Proteinuria.; Authors - Ron Korstanje
human	IHC	J Intern Med; Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans.; Authors - R Baumgartner
human	WB	Mol Oncol; Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.; Authors - Go Itoh
human	WB	PLoS Genet; A novel role for kynurenine 3-monooxygenase in mitochondrial dynamics.; Authors - Daniel C Maddison

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (03-23-2022)

works good

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Huh6 and Hepg2

KMO Antibody Western Blot validation (1/1000 dilution) in Huh6 and Hepg2 (Cat no:10698-1-AP)

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