Anticorps Polyclonal de lapin anti-KMT2C

KMT2C Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 28437-1-AP

Synonymes

MLL3, Lysine N methyltransferase 2C, KIAA1506, Homologous to ALR protein, HALR

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Galerie de données de validation

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  WB analysis using 28437-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 28437-1-AP (KMT2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 28437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28437-1-AP (KMT2C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 28437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28437-1-AP (KMT2C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562,
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

28437-1-AP cible KMT2C dans les applications de WB, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KMT2C Protéine recombinante Ag28875
• Nom complet: myeloid/lymphoid or mixed-lineage leukemia 3
• Masse moléculaire calculée: 541 kDa
• Poids moléculaire observé: 541kDa, 269 kDa
• Numéro d’acquisition GenBank: NM_170606
• Symbole du gène: KMT2C
• Identification du gène (NCBI): 58508
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lysine (K)-specific methyltransferase 2C (KMT2C, also known as MLL3) belongs to the mixed-lineage leukemia (MLL) family of histone methyltransferases which methylate the histone 3 tail at lysine 4 (H3K4) as part of the complex proteins associated with Set 1 (COMPASS) complex. Although originally identified as oncogenic fusions in leukemia, genome-wide mutation studies have revealed frequent, presumably loss-of-function, mutations in various members of the MLL family, including MLL2/KMT2B, MLL3/KMT2C, and MLL4/KMT2D in a variety of malignancies, particularly solid tumors. Mechanistic studies of KMT2C in normal cells have focused primarily on its role in enhancer regulation by deposition of H3K4me1 marks.

Publications

Species	Application	Title
human	WB	Int Immunopharmacol; Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways.; Authors - Juan-Juan Li
human	ChIP	J Biol Chem; TRIM28 represses renal cell carcinoma cell proliferation by inhibiting TFE3/KDM6A-regulated autophagy; Authors - Tanjing Song
human	WB,IF,CoIP	Adv Sci (Weinh); TAOK3 Facilitates Esophageal Squamous Cell Carcinoma Progression and Cisplatin Resistance Through Augmenting Autophagy Mediated by IRGM; Authors - Mingchuang Sun
human	WB	Nat Chem Biol; UFL1 triggers replication fork degradation by MRE11 in BRCA1/2-deficient cells; Authors - Tian Tian
Human		BMC Med Genomics; Clinical characteristics and genetic analysis of four pediatric patients with Kleefstra syndrome; Authors - Rong Ren

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