Anticorps Monoclonal anti-Kininogen 1

Kininogen 1 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

CloneNo.

10E8D11

N° de cat : 66123-1-Ig

Synonymes

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

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Galerie de données de validation

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  WB analysis of human heart using 66123-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66123-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 66123-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66123-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66123-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66123-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66123-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66123-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Kininogen 1 antibody (66123-1-Ig, Clone: 10E8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu placentaire humain, tissu plasmatique humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66123-1-Ig cible Kininogen 1 dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Kininogen 1 Protéine recombinante Ag19233
• Nom complet: kininogen 1
• Masse moléculaire calculée: 644 aa, 72 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC060039
• Symbole du gène: KNG1
• Identification du gène (NCBI): 3827
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.