Anticorps Polyclonal de lapin anti-Importin Alpha 5

• Phare
• Validé par KD/KO

Importin Alpha 5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 18137-1-AP

Synonymes

IMA5, Importin alpha 5, Importin subunit alpha 1, Impα5, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta

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Galerie de données de validation

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  WB analysis using 18137-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18137-1-AP (importin α5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18137-1-AP

  WB result of Importin Alpha 5 antibody (18137-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Alpha 5 transfected HepG2 cells.

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  IP experiment of HEK-293 using 18137-1-AP

  IP Result of anti-importin α5 (IP:18137-1-AP, 4ug; Detection:18137-1-AP 1:1000) with HEK-293 cells lysate 1480ug.

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  IHC staining of human testis using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18137-1-AP (importin α5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18137-1-AP (importin α5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 18137-1-AP

  Immunofluorescent analysis of HepG2 cells, using KPNA1 antibody 18137-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of MCF-7 using 18137-1-AP

  Immunofluorescent analysis of MCF-7 cells, using KPNA1 antibody 18137-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu testiculaire humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

18137-1-AP cible Importin Alpha 5 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Importin Alpha 5 Protéine recombinante Ag12751
• Nom complet: karyopherin alpha 1 (importin alpha 5)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC002374
• Symbole du gène: KPNA1
• Identification du gène (NCBI): 3836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.

Publications

Species	Application	Title
human,mouse	WB	Nat Immunol; Tumor-derived exosomes antagonize innate antiviral immunity.; Authors - Liang Gao
mouse	WB,IF	Acta Pharm Sin B; Activated PKB/GSK-3β synergizes with PKC-δ signaling in attenuating myocardial ischemia/reperfusion injury via potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-I.; Authors - Hao Zeng
human	WB	Nat Commun; Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.; Authors - Sergi Marco
human	WB	Nat Commun; CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.; Authors - Youzhou Sang
mouse	WB	Proc Natl Acad Sci U S A; Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.; Authors - Takahiro Kawasaki
mouse	WB	PLoS Biol; The Transcriptional Activator Krüppel-like Factor-6 Is Required for CNS Myelination.; Authors - Benjamin M Laitman ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works for WB (1:1000) and IF in permeabilized cells (1:100). Validated with siRNA

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC