Anticorps Polyclonal de lapin anti-KPNA2

• Phare
• Validé par KD/KO

KPNA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(47)

Avis (2)

N° de cat : 10819-1-AP

Synonymes

Importin subunit alpha-1, IPO A1, IPOA 1, IPOA1, Karyopherin subunit alpha-2

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Galerie de données de validation

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  WB analysis using 10819-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10819-1-AP (KPNA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10819-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10819-1-AP (KPNA2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10819-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10819-1-AP (KPNA2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10819-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KPNA2 antibody (10819-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules L02, cellules Raji
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 11 publications below
• WB: See 37 publications below
• IHC: See 15 publications below
• IF: See 8 publications below
• IP: See 4 publications below
• CoIP: See 7 publications below
• ChIP: See 1 publications below

Informations sur le produit

10819-1-AP cible KPNA2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KPNA2 Protéine recombinante Ag1153
• Nom complet: karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
• Masse moléculaire calculée: 57.8 kDa
• Poids moléculaire observé: 55-58 kDa
• Numéro d’acquisition GenBank: BC005978
• Symbole du gène: KPNA2
• Identification du gène (NCBI): 3838
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KPNA2 (karyopherin α2) is a nuclear import factor involved in the nucleocytoplasmic transport system. It is functionally involved in the tumor progression and is elevated in multiple cancers. KPNA2 expression may be a useful prognostic biomarker to monitor cancer prognosis.

Publications

Species	Application	Title
human	WB	Cell Res; Neddylation of PTEN regulates its nuclear import and promotes tumor development.; Authors - Ping Xie
human	WB,CoIP	J Exp Clin Cancer Res; ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma; Authors - Tong Xu ; KD Validated
human	WB,IP	Cell Death Differ; lncRNA THAP7-AS1, transcriptionally activated by SP1 and post-transcriptionally stabilized by METTL3-mediated m6A modification, exerts oncogenic properties by improving CUL4B entry into the nucleus.; Authors - Hai-Ting Liu
human	WB	Mol Cell Proteomics; Quantitative proteomics reveals regulation of karyopherin subunit alpha-2 (KPNA2) and its potential novel cargo proteins in nonsmall cell lung cancer.; Authors - Wang Chun-I CI ; KD Validated
human	WB,CoIP	Virol Sin; African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.; Authors - Yisha Zhuo
human	WB,IHC	Oncogene; USP1 inhibition destabilizes KPNA2 and suppresses breast cancer metastasis.; Authors - Aihui Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works for WB (1:1000) and IF in permeabilized cells (1:100). Validated with siRNA

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC

FH

Chandra (Verified Customer) (09-16-2019)

Works best at a 1:1000 antibody dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human cancer cell lines

KPNA2 Antibody Western Blot, validation (1:1000 dilution) in human cancer cell lines (Cat no:10819-1-AP)

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