Anticorps Monoclonal anti-KRAS-4A-specific

KRAS-4A-specific Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3C8F5

N° de cat : 67266-1-Ig

Synonymes

KRAS-4A, KRAS, pan RAS, 3C8F5, C K RAS

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Galerie de données de validation

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  WB analysis using 67266-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67266-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HeLa using 67266-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67266-1-Ig (KRAS-2A-specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, cellules Neuro-2a, cellules PC-12, cellules SH-SY5Y
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67266-1-Ig cible KRAS-4A-specific dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: NM_033360
• Symbole du gène: KRAS
• Identification du gène (NCBI): 3845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 4A and 4B. This antibody is specific for KRAS-4A, which is also named as KRAS-2A.

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