- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-KRAS
KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (2)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugaison
Non conjugué
N° de cat : 12063-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellules A549, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu hépatique de rat, tissu rénal de rat, tissu rénal de souris, tissu rénal humain |
Résultats positifs en IP | cellules HeLa, cellules MKN-45, tissu cérébral de souris |
Résultats positifs en IHC | tissu de cancer du poumon humain, tissu de cancer du pancréas humain, tissu rénal de souris, tissu rénal humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HeLa, |
Résultats positifs en FC (Intra) | cellules HeLa, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 15 publications below |
WB | See 140 publications below |
IHC | See 23 publications below |
IF | See 13 publications below |
IP | See 2 publications below |
Informations sur le produit
12063-1-AP cible KRAS dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, Humain, poisson-zèbre, poulet, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | KRAS Protéine recombinante Ag2700 |
Nom complet | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
Masse moléculaire calculée | 188 aa, 21 kDa |
Poids moléculaire observé | 21 kDa |
Numéro d’acquisition GenBank | BC013572 |
Symbole du gène | KRAS |
Identification du gène (NCBI) | 3845 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Protocole
Product Specific Protocols | |
---|---|
WB protocol for KRAS antibody 12063-1-AP | Download protocol |
IHC protocol for KRAS antibody 12063-1-AP | Download protocol |
IF protocol for KRAS antibody 12063-1-AP | Download protocol |
IP protocol for KRAS antibody 12063-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Biotechnol Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors. | ||
Cancer Discov Systematic functional interrogation of rare cancer variants identifies oncogenic alleles. | ||
Mol Cell The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. | ||
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sehbanul (Verified Customer) (07-24-2025) | The KRAS antibody (12063-1-AP, Proteintech) shows excellent specificity and strong performance in Western blot. It reliably detects endogenous KRAS with minimal cross-reactivity, making it a valuable tool for RAS pathway studies.
|
FH Sehbanul (Verified Customer) (07-24-2025) | The KRAS antibody (12063-1-AP, Proteintech) shows excellent specificity and strong performance in Western blot. It reliably detects endogenous KRAS with minimal cross-reactivity, making it a valuable tool for RAS pathway studies.
|
FH Anne (Verified Customer) (07-24-2025) | no comments
|
FH Sophy (Verified Customer) (12-22-2022) | I have tried the KRas antibody from a lot of companies and this one from proteintech definitely has been working the best for me.
|
FH Jonas (Verified Customer) (07-29-2022) | Antibody works as expected, dilution with 1:2000 was perfect and could even be increased to 1:5000; delivery was very fast, would recommend!!
![]() |
FH Usman Shah (Verified Customer) (11-29-2021) | Enables specific detection of KRAS in WBs.
|
FH Kishor (Verified Customer) (12-13-2018) | I am very much satisfied with this antibody. I got good results for cells and rat liver tissue protein.
|
FH Christopher (Verified Customer) (12-11-2018) | Antibody is much better than comparable antibodies. Because of weak expression an very low differences between K-Ras/H-Ras/N-Ras it's quite difficult to detect only K-Ras in a specific way. That is also the problem of this antibody. Please look at my attached blot. 1. Fermentas prestained marker2. PANC-1 whole cell lysate3. PANC-1/EGFP-K-Ras4. HEK293/EGFP-K-Ras5. HEK293/EGFP-H-Ras6.HEK293/EGFP-N-Ras7. 20 ng recombinant K-Ras (His-tagged)Unfortunately, K-Ras (12063-1-AP) recognizes also H-Ras and in less amounts N-Ras. Looking at endogenous K-Ras detection, you have to keep in mind, that the upper band is K-Ras and the lower one H-Ras. Because of the fact, that N-Ras is only recognized in very low amounts (probably almost not on endogenous level) this antibody is great detecting endogenous K-Ras in whole cell lysates with some little problems
![]() |