Anticorps Polyclonal de lapin anti-KRAS-2B-specific

• Phare
• Validé par KD/KO

KRAS-2B-specific Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 16155-1-AP

Synonymes

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2B, KRAS2B speicifc, KRAS-2B-specific, KRAS4B, Krasa, Krasb, NS3, RASK2

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Galerie de données de validation

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  WB analysis of HeLa using 16155-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16155-1-AP

  Recombinant proteins(ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS))were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16155-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16155-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16155-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16155-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 16155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16155-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, Protéine recombinante, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

16155-1-AP cible KRAS-2B-specific dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: NM_004985
• Symbole du gène: KRAS
• Identification du gène (NCBI): 3845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAS gene family encodes very similar 21KD proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2B.

Publications

Species	Application	Title
human		Nat Biotechnol; Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.; Authors - Christopher W Davies
human	FC	Nat Biomed Eng; Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.; Authors - Mahmoud Labib
human	WB	Cancer Res; Targeting eIF4A-Dependent Translation of KRAS Signaling Molecules.; Authors - Kamini Singh
human	WB	Cell Chem Biol; Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.; Authors - Sascha Röth
human	WB	Sci Signal; Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.; Authors - Andrew M Waters

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christopher (Verified Customer) (12-14-2018)

Antibody is very specific but also quite weak. Maybe you can increase the primary antibody dilution to 1:500 to increase signal. There is no unspecific recognition of H-Ras or N-Ras (see picture). Also endogenous Ras only exhibits one band and not two which is a hint for the recognition of endogenous N-Ras next to K-Ras.In immunofluorescence experiments antibody works awesome at a dilution of 1:100 for pancreatic cancer cell lines. In total I'm very satisfied, but you have to use more antibody than written in the data sheet.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras

KRAS-2B-specific Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras (Cat no:16155-1-AP)

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