Anticorps Polyclonal de lapin anti-Cytokeratin 14

Cytokeratin 14 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 22221-1-AP

Synonymes

Cell and organelle markers, CK 14, CK14, Cytokeratin 14, Cytoskeleton Marker, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

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Galerie de données de validation

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  WB analysis using 22221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 22221-1-AP

  IP Result of anti-Cytokeratin 14 (IP:22221-1-AP, 4ug; Detection:22221-1-AP 1:1000) with mouse skin tissue lysate 3200ug.

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  IHC staining of human skin using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human cervical cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 22221-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 22221-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A431 using 22221-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 14 (22221-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: tissu cutané de souris, tissu cutané de rat
• Résultats positifs en IP: tissu cutané de souris
• Résultats positifs en IHC: tissu cutané humain, tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

22221-1-AP cible Cytokeratin 14 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 14 Protéine recombinante Ag17559
• Nom complet: keratin 14
• Masse moléculaire calculée: 472 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC002690
• Symbole du gène: KRT14
• Identification du gène (NCBI): 3861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. This antibody is specifically against KRT14.

Publications

Species	Application	Title
human, hamster	WB	Cancer Gene Ther; Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model.; Authors - S Vijayalingam