Anticorps Polyclonal de lapin anti-Cytokeratin 15

Cytokeratin 15 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 10137-1-AP

Synonymes

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

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Galerie de données de validation

• 

  WB analysis of A431 using 10137-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10137-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10137-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10137-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 10137-1-AP

  IP Result of anti-Cytokeratin 15 (IP:10137-1-AP, 4ug; Detection:10137-1-AP 1:500) with A431 cells lysate 3040ug.

• 

  IHC staining of human cervical cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10137-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 10137-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 15 (10137-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules PC-3, tissu de thymus de souris
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer de l'œsophage humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 6 publications below
• IF: See 9 publications below

Informations sur le produit

10137-1-AP cible Cytokeratin 15 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 15 Protéine recombinante Ag0185
• Nom complet: keratin 15
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC002641
• Symbole du gène: KRT15
• Identification du gène (NCBI): 3866
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

Publications

Species	Application	Title
mouse	IHC	Biomater Sci; A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring; Authors - Ximeng Kang
mouse	IF	Stem Cells; LIM Homeobox Domain 2 (Lhx2) is required for corneal epithelial homeostasis.; Authors - R Sartaj
human	IF	J Invest Dermatol; GW bodies: cytoplasmic compartments in normal human skin.; Authors - Zee Jennifer M JM
human	WB	Carcinogenesis; Survival Of Skin Cancer Stem Cells Requires The Ezh2 Polycomb Group Protein.; Authors - Gautam Adhikary
mouse	IF	Sci Rep; Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.; Authors - R Sartaj
rat	WB,IF	Front Med (Lausanne); Metformin Promotes Mechanical Stretch-Induced Skin Regeneration by Improving the Proliferative Activity of Skin-Derived Stem Cells.; Authors - Shaoheng Xiong