Anticorps Polyclonal de lapin anti-Cytokeratin 16

Cytokeratin 16 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 17265-1-AP

Synonymes

CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK

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Galerie de données de validation

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  WB analysis using 17265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 17265-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17265-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17265-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 17265-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Cytokeratin 16 antibody (17265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A431 using 17265-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 16 (17265-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu cutané de souris, tissu cutané humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

17265-1-AP cible Cytokeratin 16 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 16 Protéine recombinante Ag10999
• Nom complet: keratin 16
• Masse moléculaire calculée: 473 aa, 51 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC039169
• Symbole du gène: KRT16
• Identification du gène (NCBI): 3868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

Publications

Species	Application	Title
human,mouse	WB,IF	Phytother Res; Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization; Authors - Yongling Ning
mouse	IF	Front Pharmacol; Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation; Authors - Lusheng Wang