Anticorps Polyclonal de lapin anti-Cytokeratin 17-Specific

• Phare
• Validé par KD/KO

Cytokeratin 17-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(29)

Avis (1)

N° de cat : 17516-1-AP

Synonymes

Cytokeratin 17, KRT17, CK 17, CK-17, Cytokeratin,Keratin

---

Galerie de données de validation

• 

  WB analysis using 17516-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17516-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 17516-1-AP

  IP result of anti-Cytokeratin 17-Specific (IP:17516-1-AP, 4ug; Detection:17516-1-AP 1:1000) with A431 cells lysate 1200ug.

• 

  IHC staining of human cervical squamous cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical squamous cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung squamous cell carcinoma using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung squamous cell carcinoma using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17516-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 17-Specific antibody (17516-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 17516-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 17-Specific antibody (17516-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HaCaT, cellules HeLa, tissu cutané de rat, tissu cutané de souris
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: human cervical squamous cancer tissue, human bowen disease tissue, human lung squamous cell carcinoma tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 12 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Informations sur le produit

17516-1-AP cible Cytokeratin 17-Specific dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 17
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: NM_000422
• Symbole du gène: Cytokeratin 17
• Identification du gène (NCBI): 3872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

Publications

Species	Application	Title
mouse		Science; Spatiotemporal immune zonation of the human kidney.; Authors - Benjamin J Stewart
human	IHC	Clin Cancer Res; A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer.; Authors - Yifan Wang
human	WB	Breast Cancer Res; Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation.; Authors - Dongdong Wang
human	WB	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li ; KD Validated
mouse	WB	Phytother Res; Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization; Authors - Yongling Ning
mouse,human	WB	J Invest Dermatol; Keratin 17 Impacts Global Gene Expression and Controls G2/M Cell Cycle Transition in Ionizing Radiation-Induced Skin Damage; Authors - ZhiCao Yue

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Lindsey (Verified Customer) (08-08-2025)

This antibody works very well on both FFPE tissue (with heat based citrate buffer antigen retrieval) and whole mount embryonic skin. It is highly specific based on our experience and can be used from 1/1000 to 1/1200 dilution (1/1000 for adult and 1/1200 for embryonic).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse skin

{{ptg:RelatedPrimaryAntibodies}}