Anticorps Polyclonal de lapin anti-Cytokeratin 17-Specific

• Phare
• Validé par KD/KO

Cytokeratin 17-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 17516-1-AP

Synonymes

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytokeratin 17-Specific, Cytoskeleton Marker, K17, keratin 17, KRT17, PCHC1

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Galerie de données de validation

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  WB analysis using 17516-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17516-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17516-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 17516-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 17516-1-AP

  IP Result of anti-Cytokeratin 17-Specific (IP:17516-1-AP, 4ug; Detection:17516-1-AP 1:1000) with A431 cells lysate 1200ug.

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  IHC staining of human cervical cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cervical cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 17516-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 17-Specific antibody (17516-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 17516-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 17-Specific antibody (17516-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 17516-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Cytokeratin 17-Specific antibody (17516-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, tissu cutané de souris
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:300-1:1200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 9 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

17516-1-AP cible Cytokeratin 17-Specific dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 17
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: NM_000422
• Symbole du gène: KRT17
• Identification du gène (NCBI): 3872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

Publications

Species	Application	Title
mouse		Science; Spatiotemporal immune zonation of the human kidney.; Authors - Benjamin J Stewart
human	IHC	Clin Cancer Res; A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer.; Authors - Yifan Wang
human	WB	Breast Cancer Res; Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation.; Authors - Dongdong Wang
human	WB	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li ; KD Validated
mouse	WB	Phytother Res; Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization; Authors - Yongling Ning
human,mouse	WB	J Invest Dermatol; Keratin 17 Impacts Global Gene Expression and Controls G2/M Cell Cycle Transition in Ionizing Radiation-Induced Skin Damage; Authors - ZhiCao Yue