Anticorps Polyclonal de lapin anti-Cytokeratin 18

• Phare
• Validé par KD/KO

Cytokeratin 18 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(125)

Avis (2)

N° de cat : 10830-1-AP

Synonymes

KRT18, Cell proliferation-inducing gene 46 protein, CK 18, CK18, CK-18

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Galerie de données de validation

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  WB analysis using 10830-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10830-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10830-1-AP

  WB result of Cytokeratin 18 antibody (10830-1-AP; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 18 transfected HeLa cells.

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  IP experiment of A431 using 10830-1-AP

  IP result of anti-Cytokeratin 18 (IP:10830-1-AP, 3ug; Detection:10830-1-AP 1:2000) with A431 cells lysate 3300ug.

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  IHC staining of human breast cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens).

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  IHC staining of human breast cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens).

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  IHC staining of rat liver using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human kidney using 10830-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens).

• 

  IF Staining of HeLa using 10830-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10830-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 10830-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Cytokeratin 18 Polyclonal antibody (10830-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules K-562, cellules MCF-7, cellules MDA-MB-231, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu d'amygdalite humain, tissu d'appendicite humain, tissu de cancer de l'endomètre humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du pancréas humain, tissu de cancer du poumon humain, tissu de carcinome à cellules rénales humain, tissu de tumeur ovarienne humain, tissu d'hyperplasie de la prostate humain, tissu hépatique de rat, tissu hépatique de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 40 publications below
• IHC: See 27 publications below
• IF: See 62 publications below

Informations sur le produit

10830-1-AP cible Cytokeratin 18 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, porc, singe, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 18 Protéine recombinante Ag1260
• Nom complet: keratin 18
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000180
• Symbole du gène: Cytokeratin 18
• Identification du gène (NCBI): 3875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Publications

Species	Application	Title
mouse	IHC	Cell Stem Cell; Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.; Authors - Brian Lin
mouse	IF	Hepatology; Fat-associated Lymphoid Clusters as Expandable Niches for Ectopic Liver Development.; Authors - Bing Han
rat	IHC	J Control Release; Mesenchymal stem cell exosomes as nanotherapeutics for dry age-related macular degeneration; Authors - Yue Tang
rat	IF	Acta Biomater; A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon.; Authors - Yingzheng Zhao
mouse	IF	Proc Natl Acad Sci U S A; Basal epithelial stem cells are efficient targets for prostate cancer initiation.; Authors - Lawson Devon A DA
mouse	IHC,IF	Proc Natl Acad Sci U S A; Role of estrogen receptor beta in uterine stroma and epithelium: Insights from estrogen receptor beta-/- mice.; Authors - Wada-Hiraike Osamu O

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jan (Verified Customer) (11-20-2024)

Photo attached represents IF analysis of fixed A549 cells. Cells were incubated with Cytokeratin 18 polyclonal antibody (10830-1-AP) and FITC-conjugated Goat Anti-Rabbit IgG (H+L) (SA00003-2).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: A549

Cytokeratin 18 Antibody Immunofluorescence validation (1:200 dilution) in A549 (Cat no:10830-1-AP)

FH

Parmveer (Verified Customer) (12-02-2019)

Works well for both IHC and IF, but does come with a bit of background

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate

Cytokeratin 18 Antibody Immunohistochemistry,Immunofluorescence, validation (1:1000 dilution) in Prostate (Cat no:10830-1-AP)

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