Anticorps Polyclonal de lapin anti-Cytokeratin 19

• Phare
• Validé par KD/KO

Cytokeratin 19 Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 14965-1-AP

Synonymes

CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 14965-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14965-1-AP

  WB result of KRT19 antibody (14965-1-AP, 1:50,000) with si-Control and si-KRT19 transfected HeLa cells.

• 

  WB analysis of HepG2 using 14965-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14965-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14965-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse liver using 14965-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using Cytokeratin 19 antibody (14965-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 14965-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytokeratin 19 antibody (14965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 14965-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 19 antibody (14965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  FC experiment of MCF-7 using 14965-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug Cytokeratin 19 Polyclonal antibody (14965-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules MCF7, cellules BxPC-3, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du col de l'utérus humain, tissu de cancer du foie humain, tissu de cancer du poumon humain, tissu de côlon de rat, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-Fro: tissu hépatique de souris,
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 8 publications below
• IF: See 8 publications below

Informations sur le produit

14965-1-AP cible Cytokeratin 19 dans les applications de WB, IHC, IF/ICC, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 19 Protéine recombinante Ag6830
• Nom complet: keratin 19
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 40 kDa, 46 kDa
• Numéro d’acquisition GenBank: BC002539
• Symbole du gène: Cytokeratin 19
• Identification du gène (NCBI): 3880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19.

Publications

Species	Application	Title
mouse	IF	Gastroenterology; ECM1 Prevents Activation of Transforming Growth Factor β, Hepatic Stellate Cells, and Fibrogenesis in Mice.; Authors - Weiguo Fan
mouse	IF	Hepatology; Cholestasis-induced adaptive remodeling of interlobular bile ducts.; Authors - Nachiket Vartak
monkey	IHC,IF	Int J Biol Sci; Hepatobiliary organoids differentiated from hiPSCs relieve cholestasis-induced liver fibrosis in nonhuman primates; Authors - Hongmei Li
human	IHC	Cell Rep; In Vivo Functional Platform Targeting Patient-Derived Xenografts Identifies WDR5-Myc Association as a Critical Determinant of Pancreatic Cancer.; Authors - Alessandro Carugo
mouse	IHC	Arch Toxicol; Galunisertib modifies the liver fibrotic composition in the Abcb4Ko mouse model.; Authors - Seddik Hammad
mouse	IF	Front Cell Dev Biol; Volumetric imaging reveals VEGF-C-dependent formation of hepatic lymph vessels in mice; Authors - Stefanie Bobe

{{ptg:RelatedPrimaryAntibodies}}