Anticorps Polyclonal de lapin anti-Cytokeratin 20

Cytokeratin 20 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 17329-1-AP

Synonymes

Cell and organelle markers, CK 20, CK20, Cytokeratin 20, Cytoskeleton Marker, K20, keratin 20, KRT20, KRT21, Protein IT

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Galerie de données de validation

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  WB analysis using 17329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17329-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17329-1-AP (Cytokeratin 20 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17329-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 17329-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HT-29 using 17329-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Rabbit colon using 17329-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Rabbit colon tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Rabbit colon using 17329-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Rabbit colon tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 17329-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HT-29 using 17329-1-AP

  1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 20 (17329-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules HT-29, cellules MCF-7
• Résultats positifs en IHC: tissu de côlon humain, tissu d'appendicite humain, tissu de cancer de l'estomac humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HT-29, cellules HeLa
• Résultats positifs en cytométrie: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:1000-1:4000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Informations sur le produit

17329-1-AP cible Cytokeratin 20 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 20 Protéine recombinante Ag11308
• Nom complet: keratin 20
• Masse moléculaire calculée: 424 aa, 48 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC031559
• Symbole du gène: KRT20
• Identification du gène (NCBI): 54474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Publications

Species	Application	Title
mouse	IF	Cell Res; Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons.; Authors - Pingping Zhu
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
		Neuron; 5-hydroxytryptamine produced by enteric serotonergic neurons initiates colorectal cancer stem cell self-renewal and tumorigenesis.; Authors - Pingping Zhu
human	IF	Biomaterials; A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium.; Authors - Yuli Wang
mouse	IF	Cancer Lett; LGR4 cooperates with PrPc to endow the stemness of colorectal cancer stem cells contributing to tumorigenesis and liver metastasis.; Authors - Qi Cheng
mouse	IHC	Nutrients; Propolis Has an Anticancer Effect on Early Stage Colorectal Cancer by Affecting Epithelial Differentiation and Gut Immunity in the Tumor Microenvironment; Authors - Ming-Hung Shen

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