Anticorps Polyclonal de lapin anti-KRT33B

KRT33B Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 11048-1-AP

Synonymes

Ha 3II, HA3II, Hair keratin, type I Ha3 II, hHa3 II, HKA3B, K33B, keratin 33B, KRT33B, KRTHA3A, KRTHA3B

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Galerie de données de validation

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  WB analysis of MCF-7 using 11048-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11048-1-AP (KRT33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11048-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11048-1-AP (KRT33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11048-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 11048-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11048-1-AP (KRT33B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

11048-1-AP cible KRT33B dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KRT33B Protéine recombinante Ag1522
• Nom complet: keratin 33B
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC009971
• Symbole du gène: KRT33B
• Identification du gène (NCBI): 3884
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 33 belongs to type I keratin and is found in hair and nails. There are two isoforms of this protein, encoded by two separate genes, KRTHA3A and KRTHA3B.

Publications

Species	Application	Title
human	WB	Asian J Androl; PMID: 24994782; Authors - Yan-Min Ma