Anticorps Polyclonal de lapin anti-Cytokeratin 7

Cytokeratin 7 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 15539-1-AP

Synonymes

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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Galerie de données de validation

• 

  WB analysis using 15539-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15539-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15539-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15539-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15539-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HeLa using 15539-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 7 antibody (15539-1-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15539-1-AP

  1X10^6 HeLa cells were stained with 0.2 ug Anti-Human Cytokeratin 7 (15539-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

• 

  FC experiment of HeLa using 15539-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Cytokeratin 7 antibody (15539-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HepG2, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu pancréatique humain, tissu de cancer de l'estomac humain, tissu de cancer du foie humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu de tumeur ovarienne humain, tissu pulmonaire humain, tissu rénal de rat, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:30000
• Immunofluorescence (IF): IF : 1:350-1:1400
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 8 publications below
• IF: See 9 publications below

Informations sur le produit

15539-1-AP cible Cytokeratin 7 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 7 Protéine recombinante Ag7895
• Nom complet: keratin 7
• Masse moléculaire calculée: 469 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC002700
• Symbole du gène: KRT7
• Identification du gène (NCBI): 3855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Publications

Species	Application	Title
	IF	Cell; Neurulation of the cynomolgus monkey embryo achieved from 3D blastocyst culture; Authors - Jinglei Zhai
mouse	IHC	Cancer Cell; Promotion of cholangiocarcinoma growth by diverse cancer-associated fibroblast subpopulations.; Authors - Silvia Affo
human	IF	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
human	WB, IHC, IF	Cell Death Dis; LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.; Authors - S Liu
rat	IHC	J Ethnopharmacol; Astragaloside prevents BDL-induced liver fibrosis through inhibition of notch signaling activation.; Authors - Mu Yongping
human	IF	Stem Cell Res Ther; CCL20/CCR6 axis mediates macrophages to promote proliferation and migration of ESCs by blocking autophagic flux in endometriosis; Authors - Jiahuan Tan

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