Anticorps Polyclonal de lapin anti-KRT80

• Phare
• Validé par KD/KO

KRT80 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 16835-1-AP

Synonymes

CK 80, Cytokeratin 80, K80, KB20, keratin 80, KRT80, Type II keratin Kb20

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 16835-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16835-1-AP (KRT80 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 16835-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16835-1-AP (KRT80 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16835-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16835-1-AP (KRT80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3600
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Informations sur le produit

16835-1-AP cible KRT80 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KRT80 Protéine recombinante Ag10498
• Nom complet: keratin 80
• Masse moléculaire calculée: 422 aa, 47 kDa
• Poids moléculaire observé: 47 kDa, 50 kDa
• Numéro d’acquisition GenBank: BC065180
• Symbole du gène: KRT80
• Identification du gène (NCBI): 144501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KRT80 is a human IF type II epithelial keratin gene involved in the formation of IF heterodimers in various epithelial cells. KRT80 is overexpressed in many neoplasms and plays an essential role in promoting cell proliferation, migration and invasiveness, and is associated with poor prognosis in cancer patients. KRT80 has three isoforms with predicted MW of 50 kDa, 47 kDa, and 54 kDa.

Publications

Species	Application	Title
human	WB	Mol Cell; Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.; Authors - Daniel Arango
human	IHC	Cell Discov; Single-cell atlas of keratoconus corneas revealed aberrant transcriptional signatures and implicated mechanical stretch as a trigger for keratoconus pathogenesis.; Authors - Shengqian Dou
human	IF	Int J Mol Sci; Keratin Profiling by Single-Cell RNA-Sequencing Identifies Human Prostate Stem Cell Lineage Hierarchy and Cancer Stem-Like Cells.; Authors - Wen-Yang Hu
human	WB,IF	Cell Death Dis; Keratin 80 promotes migration and invasion of colorectal carcinoma by interacting with PRKDC via activating the AKT pathway.; Authors - Changcan Li
human	WB	Cell Cycle; MiR-4268 suppresses gastric cancer genesis through inhibiting keratin 80.; Authors - Fan Zhang ; KD Validated
human	WB,IHC	J Cancer; Keratin 80 regulated by miR-206/ETS1 promotes tumor progression via the MEK/ERK pathway in ovarian cancer.; Authors - Ouxuan Liu