Anticorps Polyclonal de lapin anti-KU70,XRCC6

• Phare
• Validé par KD/KO

KU70,XRCC6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (1)

N° de cat : 10723-1-AP

Synonymes

5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, ML8, Thyroid lupus autoantigen, TLAA, XRCC6

---

Galerie de données de validation

• 

  WB analysis using 10723-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10723-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10723-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10723-1-AP

  IP Result of anti-KU70,XRCC6 (IP:10723-1-AP, 3ug; Detection:10723-1-AP 1:1000) with HeLa cells lysate 1600ug.

• 

  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat testis using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 10723-1-AP

  Immunofluorescent analysis of HepG2 cells, using KU70,XRCC6 antibody 10723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 10723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KU70 antibody (10723-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10723-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug KU70 antibody (10723-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HeLa, cellules MCF-7, tissu de côlon de souris, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu rénal de souris, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10723-1-AP cible KU70,XRCC6 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KU70,XRCC6 Protéine recombinante Ag1043
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 6
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC008343
• Symbole du gène: XRCC6
• Identification du gène (NCBI): 2547
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

Publications

Species	Application	Title
human	WB,IF	Oncogene; UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.; Authors - L Hu
human	WB	Phytomedicine; Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer; Authors - Meiyang Chen
human	WB	J Mater Chem B; Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer; Authors - Shuying Li
human	WB	FEBS J; Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy; Authors - Yan Wei
rat	WB	FASEB J; Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.; Authors - Catherine E Aiken
human	WB	Front Oncol; ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway; Authors - Xin Wen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Jonathan (Verified Customer) (02-03-2019)

Worked well for Western Blotting with little background.

• Applications: Western Blot,