Anticorps Polyclonal de lapin anti-KU70,XRCC6

• Phare
• Validé par KD/KO

KU70,XRCC6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(39)

Avis (1)

N° de cat : 10723-1-AP

Synonymes

KU70, XRCC6, 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70, ATP-dependent DNA helicase 2 subunit 1

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Galerie de données de validation

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  WB analysis using 10723-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10723-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10723-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10723-1-AP

  IP result of anti-KU70,XRCC6 (IP:10723-1-AP, 3ug; Detection:10723-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10723-1-AP (KU70,XRCC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10723-1-AP

  Immunofluorescent analysis of HepG2 cells, using KU70,XRCC6 antibody 10723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 10723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KU70,XRCC6 antibody (10723-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 10723-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KU70,XRCC6 Polyclonal antibody (10723-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HeLa, cellules MCF-7, tissu de côlon de souris, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu rénal de souris, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 35 publications below
• IHC: See 3 publications below
• IF: See 9 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

10723-1-AP cible KU70,XRCC6 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KU70,XRCC6 Protéine recombinante Ag1043
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 6
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC008343
• Symbole du gène: KU70
• Identification du gène (NCBI): 2547
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

Publications

Species	Application	Title
		Mol Cell; A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis; Authors - Kaiwen Bao
human	WB,IF	Oncogene; UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.; Authors - L Hu
human	WB	Phytomedicine; Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer; Authors - Meiyang Chen
human	WB	J Mater Chem B; Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer; Authors - Shuying Li
human	IF	Mol Med; Nuclear VPS35 attenuates NHEJ repair by sequestering Ku protein; Authors - Luping Zhang
human	WB	FEBS J; Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy; Authors - Yan Wei

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jonathan (Verified Customer) (02-03-2019)

Worked well for Western Blotting with little background.

• Applications: Western Blot,

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