Anticorps Recombinant de lapin anti-Kininogen 1

Kininogen 1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241397C11

N° de cat : 84175-5-RR

Synonymes

KNG1, 241397C11, Alpha-2-thiol proteinase inhibitor, BDK, Bradykinin

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Galerie de données de validation

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  WB analysis of human plasma using 84175-5-RR

  Human plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HT-29 using 84175-5-RR

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Kininogen-1 Recombinant antibody (84175-5-RR, Clone:241397C11) (red), or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84175-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84175-5-RR against Human Kininogen-1 were performed. The affinity constant is 0.182 nM.

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Applications testées

• Résultats positifs en WB: plasma humain,
• Résultats positifs en FC (Intra): cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84175-5-RR cible Kininogen 1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: kininogen 1
• Masse moléculaire calculée: 72kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: NM_001102416.3
• Symbole du gène: Kininogen 1
• Identification du gène (NCBI): 3827
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

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