Anticorps Polyclonal de lapin anti-L2HGDH

• Phare
• Validé par KD/KO

L2HGDH Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (2)

N° de cat : 15707-1-AP

Synonymes

C14orf160, DURANIN, L2HGDH

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Galerie de données de validation

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  WB analysis using 15707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15707-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH Antibody) at dilution of 1:800 incubated at 4 degree celsius over night.

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  WB analysis of mouse small intestine using 15707-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 15707-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15707-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15707-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15707-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15707-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules MCF-7, cellules SGC-7901, tissu cérébral de rat, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 13 publications below
• IHC: See 1 publications below

Informations sur le produit

15707-1-AP cible L2HGDH dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: L2HGDH Protéine recombinante Ag8382
• Nom complet: L-2-hydroxyglutarate dehydrogenase
• Masse moléculaire calculée: 463aa,50 kDa; 441aa,49 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC006117
• Symbole du gène: L2HGDH
• Identification du gène (NCBI): 79944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.

Publications

Species	Application	Title
human	WB	Cell Metab; Hypoxia Induces Production of L-2-Hydroxyglutarate.; Authors - Andrew M Intlekofer ; KD Validated
human	WB	Cell Metab; Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.; Authors - William M Oldham ; KD Validated
human	WB	Sci Transl Med; 2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity.; Authors - Parker L Sulkowski ; KD Validated
human	WB	Cancer Discov; L-2-Hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer.; Authors - Eun-Hee Shim
human	WB	Cell Chem Biol; MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.; Authors - ZhiJun Qiu ; KO Validated
		Clin Cancer Res; Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer.; Authors - Sandeep Shelar

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anirban (Verified Customer) (12-05-2021)

24 ug protein/lane. One non specific band observed above the KD band. 5% milk was used to block (1 hr incubation) and in secondary Ab (1/5000 dilution-45 minutes incubation). Primary Ab was diluted in 3% BSA (1.5 hrs incubation. All were done in TBST (0.1% Tween 20). Nitrocellulose membrane was used.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Renca (mouse renal cancer cell line)

L2HGDH Antibody Western Blot validation (1/1000 dilution) in Renca (mouse renal cancer cell line) (Cat no:15707-1-AP)

FH

Daniel (Verified Customer) (09-30-2019)

Tissue was boiled in SDS-sample buffer and separated by size on an SDS-PAGE.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver

L2HGDH Antibody Western Blot, validation (1:1000 dilution) in Mouse liver (Cat no:15707-1-AP)