Anticorps Polyclonal de lapin anti-LAMA4 (Isoform 3)

LAMA4 (Isoform 3) Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 10465-1-AP

Synonymes

LAMA4 (Isoform 3), Laminin 14 subunit alpha, Laminin 8 subunit alpha, Laminin 9 subunit alpha, Laminin subunit alpha 4, laminin, alpha 4

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Galerie de données de validation

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  WB analysis of human heart using 10465-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10465-1-AP (LAMA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 10465-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10465-1-AP (LAMA4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 10465-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10465-1-AP (LAMA4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 10465-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10465-1-AP (LAMA4 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 6 publications below

Informations sur le produit

10465-1-AP cible LAMA4 (Isoform 3) dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LAMA4 (Isoform 3) Protéine recombinante Ag0736
• Nom complet: laminin, alpha 4
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 11-13 kDa
• Numéro d’acquisition GenBank: BC004241
• Symbole du gène: Laminin alpha 4/LAMA4
• Identification du gène (NCBI): 3910
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2 (PMID: 7959779). LAMA4 (laminin, alpha 4), an A chain variant, is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423) (PMID: 10842354; 10964957). LAMA4 is widely distributed in developing and adult human tissues, and mainly localized to mesenchyme-derived tissues (PMID: 12133914). It may have a role in formation and function of endothelium, transmigration of inflammatory cells through endothelium, and invasion of certain tumors (PMID: 17533363). Three alternatively spliced mRNAs give rise to three isoforms. This antibody was generated against the full length (1-120aa) of isoform 3.

Publications

Species	Application	Title
human	IHC	Arthritis Rheum; PMID: 21337318; Authors - Gutierrez-Roelens I I
human	IHC	Sci Rep; Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.; Authors - Katsutaka Mitachi
mouse	IHC	Am J Physiol Heart Circ Physiol; Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.; Authors - Li Lin
human	IHC	Neuromuscul Disord; Basement membrane remodelling and segmental fibrosis in sporadic inclusion body myositis.; Authors - Doppler K K
human	IHC	J Neurosci Res; Maturational changes in laminin, fibronectin, collagen IV, and perlecan in germinal matrix, cortex, and white matter and effect of betamethasone.; Authors - Xu Hongmin H
human	WB,IHC	Cancer Cell Int; MiR-539 inhibits proliferation and migration of triple-negative breast cancer cells by down-regulating LAMA4 expression.; Authors - Zhi-Xue Yang

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