Anticorps Monoclonal anti-CD107b / LAMP2

• Phare
• Validé par KD/KO

CD107b / LAMP2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgA

Réactivité testée

Humain, porc, souris et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

81

CloneNo.

2D3B9

Avis (5)

N° de cat : 66301-1-Ig

Synonymes

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

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Galerie de données de validation

• 

  WB analysis using 66301-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66301-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66301-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 66301-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66301-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66301-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66301-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66301-1-Ig (CD107b / LAMP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66301-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66301-1-Ig (CD107b / LAMP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66301-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66301-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66301-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66301-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 66301-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66301-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 66301-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66301-1-Ig (LAMP2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), phalloidine stains F-actin (red).

• 

  IF Staining of HeLa using 66301-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66301-1-Ig (LAMP2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), phalloidine stains F-actin (red).

• 

  IF Staining of HepG2 using 66301-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66301-1-Ig(LAMP2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66301-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66301-1-Ig (LAMP2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of 3T3-L1 using 66301-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed 3T3-L1 cells using 66301-1-Ig (LAMP2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HUVEC, cellules Jurkat, tissu hépatique de porc, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du poumon humain, tissu hépatique de souris, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules 3T3-L1, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 53 publications below
• IHC: See 2 publications below
• IF: See 40 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

66301-1-Ig cible CD107b / LAMP2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: bovin, Humain, porc, poulet, souris
• Hôte / Isotype: Mouse / IgA
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD107b / LAMP2 Protéine recombinante Ag0595
• Nom complet: lysosomal-associated membrane protein 2
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC002965
• Symbole du gène: LAMP2
• Identification du gène (NCBI): 3920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.

Publications

Species	Application	Title
mouse	WB	Nature; Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.; Authors - Xinjian Liu
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	IF	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
	WB	Arthritis Rheumatol; LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands; Authors - Hiroyuki Nakamura
human	IF	J Clin Invest; TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.; Authors - Haiqing Zhang
mouse	WB	Redox Biol; WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury; Authors - Zebin Deng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

LENIN (Verified Customer) (06-12-2022)

Antibody works very well for culture cells and Western blots.

• Applications: Cell culture
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: LIVER CELLS

CD107b / LAMP2 Antibody Cell culture validation (1:1000 dilution) in LIVER CELLS (Cat no:66301-1-Ig)

FH

NYDIA (Verified Customer) (03-12-2022)

This antibody works great for immunofluorescence. No background. I highly recommend this product.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa cells

FH

Eric (Verified Customer) (03-13-2021)

I had success with some and others not. I used microglia and I did not get strong expression but in macrophages I got better bands with same dilution and treatment. Many nonspecific bands

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Microglia/Macrophages

CD107b / LAMP2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Microglia/Macrophages (Cat no:66301-1-Ig)

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

miguel (Verified Customer) (12-11-2018)

The antibody was used with an inhouse protocol for Immunohistochemistry. The results varied from good to great for both stains and imaging.

• Applications: Western Blot, Immunohistochemistry,
• Cell Tissue Type: Rat Brain

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