Anticorps Polyclonal de lapin anti-LARP7

• Phare
• Validé par KD/KO

LARP7 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 17067-1-AP

Synonymes

HDCMA18P, La related protein 7, LARP7, PIP7S

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Galerie de données de validation

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  WB analysis using 17067-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17067-1-AP (LARP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17067-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17067-1-AP (LARP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17067-1-AP (LARP7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 17067-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17067-1-AP (LARP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17067-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17067-1-AP (LARP7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules NIH/3T3, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cutané humain, tissu de cancer de l'estomac humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

17067-1-AP cible LARP7 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LARP7 Protéine recombinante Ag10891
• Nom complet: La ribonucleoprotein domain family, member 7
• Masse moléculaire calculée: 582 aa, 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC006981
• Symbole du gène: LARP7
• Identification du gène (NCBI): 51574
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LARP7 belongs to the LARP RNA-binding protein family, which includes the lupus LA antigen gene, and modulates the metabolism and function of a variety of RNA species [PMID:20138158]. LARP7 binds to the 7SK RNP complex, and sequesters the positive transcription elongation factor b (P-TEFb) in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. binds to and stabilizes 7sk snRNA[PMID:19416841].

Publications

Species	Application	Title
mouse	WB	Genes Dev; Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells.; Authors - Daiji Okamura
mouse,human	IF,IP	Nat Commun; Interaction of 7SK with the Smn complex modulates snRNP production.; Authors - Changhe Ji ; KD Validated
human	WB	Nucleic Acids Res; 7SK small nuclear RNA directly affects HMGA1 function in transcription regulation.; Authors - Eilebrecht Sebastian S
human	WB	Nucleic Acids Res; HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.; Authors - Sebastian Eilebrecht
human	WB	EMBO Rep; Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs.; Authors - Ahmed S Warda
human	WB,IF,IP	MBio; Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.; Authors - Juanita Von Dwingelo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Monika (Verified Customer) (10-09-2023)

Great antibody, successfully used in western blots with 1:1000 to 1:2000 dilutions. Verified using a Calu-3 and HEK293T LARP7 knock-out cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: lung carcinoma, Calu-3 cells

LARP7 Antibody Western Blot validation (1:1000 dilution) in lung carcinoma, Calu-3 cells (Cat no:17067-1-AP)