Anticorps Polyclonal de lapin anti-L-Plastin

• Phare
• Validé par KD/KO

L-Plastin Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 13025-1-AP

Synonymes

LCP1, L PLASTIN, LCP 1, LCP-1, LPL

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Galerie de données de validation

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  WB analysis of Jurkat using 13025-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13025-1-AP (L-Plastin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13025-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13025-1-AP (L-Plastin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13025-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13025-1-AP (L-Plastin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13025-1-AP

  IP result of anti-L-Plastin (IP:13025-1-AP, 4ug; Detection:13025-1-AP 1:1000) with Jurkat cells lysate 2400ug.

• 

  IHC staining of human lymphoma using 13025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13025-1-AP (L-Plastin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 13025-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13025-1-AP (L-Plastin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 13025-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13025-1-AP (L-Plastin antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, tissu splénique de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de lymphome humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

13025-1-AP cible L-Plastin dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, singe, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: L-Plastin Protéine recombinante Ag3571
• Nom complet: lymphocyte cytosolic protein 1 (L-plastin)
• Masse moléculaire calculée: 627 aa, 64 kDa
• Poids moléculaire observé: 64-70 kDa
• Numéro d’acquisition GenBank: BC010271
• Symbole du gène: L-Plastin
• Identification du gène (NCBI): 3936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LCP1, also known as L-Plastin, is a member of the Plastin gene family. The family members include L-Plastin (LCP1), T-Plastin (PLS3), and I-Plastin (PLS1), which can regulate the dynamic reorganization of the cytoskeleton by binding and cross-linking actin filaments. In lymphocytes, LCP1 can regulate immune responses, especially during inflammation and infection. It acts as a key regulator of T-cell activation by responding to co-stimulatory signals through TCR/CD3 and CD2 or CD28, and regulates the cell-surface expression of IL2RA/CD25 and CD69 (PMID: 17294403).

Publications

Species	Application	Title
human	WB	Sci Signal; A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity; Authors - Hyunbae Kim
human	WB,IHC	Mol Ther Nucleic Acids; Exosomal Transfer of LCP1 Promotes Osteosarcoma Cell Tumorigenesis and Metastasis by Activating the JAK2/STAT3 Signaling Pathway.; Authors - Xuhui Ge
zebrafish	IF	J Cell Sci; Iterative Bleaching Extends Multiplexity (IBEX) facilitates simultaneous identification of all major retinal cell types; Authors - Aanandita A Kothurkar
human	IHC	Cancer Biomark; LCP1 is a prognostic biomarker correlated with immune infiltrates in gastric cancer; Authors - Qingwen Zeng
mouse	WB	Drug Dev Res; Therapeutic role of Artemether in the prevention of hepatic steatosis through miR-34a-5p/PPARα pathway; Authors - Li Chen
monkey	IHC	Mol Biosyst; Unravelling the proteome of adult rhesus monkey ovaries.; Authors - Hui He

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