Anticorps Polyclonal de lapin anti-LDHB

• Phare
• Validé par KD/KO

LDHB Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

47

N° de cat : 14824-1-AP

Synonymes

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, LDH-B, TRG 5

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Galerie de données de validation

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  WB analysis using 14824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14824-1-AP (LDHB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14824-1-AP

  WB result of LDHB antibody (14824-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDHB transfected HeLa cells.

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  IP experiment of mouse brain using 14824-1-AP

  IP Result of anti-LDHB (IP:14824-1-AP, 3ug; Detection:14824-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 14824-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14824-1-AP (LDHB antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human kidney using 14824-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14824-1-AP (LDHB antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human prostate cancer using 14824-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14824-1-AP (LDHB Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14824-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14824-1-AP (LDHB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14824-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDHB antibody (14824-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 14824-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LDHB antibody (14824-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules DU 145, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 38 publications below
• IHC: See 8 publications below
• IF: See 6 publications below

Informations sur le produit

14824-1-AP cible LDHB dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LDHB Protéine recombinante Ag6605
• Nom complet: lactate dehydrogenase B
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC002362
• Symbole du gène: LDHB
• Identification du gène (NCBI): 3945
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway. LDH comprises LDHA and LDHB, two subunits that are encoded by independent genes. In muscle or liver, most of the LDH is composed of four LDHA subunits, and preferably catalyzes the reduction of pyruvate to lactate. In heart and brain, LDH is mainly composed of four LDHB subunits, and predominantly catalyzes the oxidation of lactate to pyruvate. In other tissues, LDH is composed of both LDHA and LDHB. (PMID: 26269128, 31804482)

Publications

Species	Application	Title
human	WB	Nat Methods; A large-scale targeted proteomics assay resource based on an in vitro human proteome.; Authors - Masaki Matsumoto
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	Nucleic Acids Res; Histone lactylation-boosted ALKBH3 potentiates tumor progression and diminished promyelocytic leukemia protein nuclear condensates by m1A demethylation of SP100A; Authors - Xiang Gu
mouse	WB	Nat Commun; Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.; Authors - Jin-Ho Koh
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
mouse	WB	Nat Commun; LDHA-mediated ROS generation in chondrocytes is a potential therapeutic target for osteoarthritis.; Authors - Manoj Arra