Anticorps Monoclonal anti-LDHB

LDHB Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

5E9G1

Avis (1)

N° de cat : 66425-1-Ig

Synonymes

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, LDH-B, TRG 5

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Galerie de données de validation

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  WB analysis using 66425-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66425-1-Ig (LDHB antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IHC staining of mouse heart using 66425-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66425-1-Ig (LDHB antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 66425-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66425-1-Ig (LDHB antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66425-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66425-1-Ig (LDHB antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Neuro-2a, cellules PC-12, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:16000-1:64000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

66425-1-Ig cible LDHB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LDHB Protéine recombinante Ag6605
• Nom complet: lactate dehydrogenase B
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC002362
• Symbole du gène: LDHB
• Identification du gène (NCBI): 3945
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.

Publications

Species	Application	Title
human,mouse	WB	Acta Pharm Sin B; Co-delivery of photosensitizer and diclofenac through sequentially responsive bilirubin nanocarriers for combating hypoxic tumors.; Authors - Yang Zhou
mouse	WB	Life Sci; Aerobic exercise reduced the amount of CHRONO bound to BMAL1 and ameliorated glucose metabolic dysfunction in skeletal muscle of high-fat diet-fed mice; Authors - Lei Xu
rat	WB,IF	Cell Death Dis; PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming.; Authors - Wen-Qi Ma
mouse	WB	Life (Basel); Skeletal-Muscle-Specific Overexpression of Chrono Leads to Disruption of Glucose Metabolism and Exercise Capacity; Authors - Shiyi He

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reema (Verified Customer) (09-07-2022)

The antibody worked really good with western blot. I would refer this to anyone who is doing western blotting with rat tissue samples.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: rat

LDHB Antibody Western Blot validation (1:1000 dilution) in rat (Cat no:66425-1-Ig)