Anticorps Polyclonal de lapin anti-LDHC-Specific

LDHC-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 19989-1-AP

Synonymes

Cancer/testis antigen 32, CT32, lactate dehydrogenase C, LDH C, LDH testis subunit, LDH X, LDH3, LDHC, LDHC-Specific, LDHX

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Galerie de données de validation

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  WB analysis of human testis using 19989-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 19989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19989-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19989-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19989-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 19989-1-AP

  IP Result of anti-LDHC-Specific (IP:19989-1-AP, 4ug; Detection:19989-1-AP 1:500) with mouse testis tissue lysate 2400ug.

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  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human heart using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19989-1-AP

  Immunofluorescent analysis of Hela cells, using LDHC antibody 19989-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules A549, tissu cérébral de souris, tissu pulmonaire de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cardiaque humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

19989-1-AP cible LDHC-Specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lactate dehydrogenase C
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 30-36 kDa
• Numéro d’acquisition GenBank: NM_002301
• Symbole du gène: LDHC
• Identification du gène (NCBI): 3948
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis.; Authors - Yuping Chen
	IHC	Med Sci Monit; PMID: 31332157; Authors - Li Yang
mouse	WB,IHC	Andrology; Generation of humanized LDHC knock-in mice as a tool to assess human LDHC-targeting contraceptive drugs; Authors - Rie Iida-Norita

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (09-23-2023)

Utilizing the concentration of 1:1000, WB results are clear in kinds lung cancer cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lung cancer cell lines

LDHC-Specific Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines (Cat no:19989-1-AP)