Anticorps Polyclonal de lapin anti-LEPR

• Phare
• Validé par KD/KO

LEPR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 20966-1-AP

Synonymes

CD295, DB, HuB219, LEP R, LEPR, leptin receptor, OB R, OB receptor, OBR

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Galerie de données de validation

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  WB analysis using 20966-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20966-1-AP (LEPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 20966-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20966-1-AP (LEPR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 20966-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20966-1-AP (LEPR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 20966-1-AP

  IP Result of anti-LEPR (IP:20966-1-AP, 5ug; Detection:20966-1-AP 1:700) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse liver using 20966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20966-1-AP (LEPR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20966-1-AP (LEPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 20966-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20966-1-AP (LEPR antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 20966-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20966-1-AP (LEPR antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 20966-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20966-1-AP (LEPR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20966-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20966-1-AP (LEPR antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 20966-1-AP

  Immunofluorescent analysis of MCF-7 cells using 20966-1-AP (LEPR antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HeLa using 20966-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LEPR antibody (20966-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules PC-3, tissu cardiaque de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu hépatique de souris, tissu cardiaque humain, tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

20966-1-AP cible LEPR dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: leptin receptor
• Masse moléculaire calculée: 132 kDa
• Poids moléculaire observé: 100-150 kDa
• Numéro d’acquisition GenBank: NM_002303
• Symbole du gène: LEPR
• Identification du gène (NCBI): 3953
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction (PMID: 22249808). Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3 (PMID: 9102398). LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity. Alternatively spliced transcript variants encoding different isoforms of LEPR have been described. This antibody can recognize long (LEPRb) and short (LEPRa,c,d) isoforms. The band at 150 kDa detected by this antibody is larger than the predicted molecular weight, probably due to glycosylation (PMID: 10026237).

Publications

Species	Application	Title
		Cancer Lett; Pharmacological inhibition of KDM1A/LSD1 enhances estrogen receptor beta-mediated tumor suppression in ovarian cancer; Authors - Prabhakar Pitta Venkata
human	WB, IHC	Oncotarget; Leptin signaling enhances cell invasion and promotes the metastasis of human pancreatic cancer via increasing MMP-13 production.; Authors - Yingchao Fan
human	IHC	Cancers (Basel); Knockdown of Leptin Receptor Affects Macrophage Phenotype in the Tumor Microenvironment Inhibiting Breast Cancer Growth and Progression.; Authors - Luca Gelsomino
human	WB	Comput Struct Biotechnol J; Celastrol inhibits the proliferation and migration of MCF-7 cells through the leptin-triggered PI3K/AKT pathway.; Authors - Pingping Chen
mouse	WB	FASEB J; Immunotherapy against angiotensin II receptor ameliorated insulin resistance in a leptin receptor-dependent manner.; Authors - Jiayu Zheng ; KD Validated
human	IHC	Biomedicines; Leptin, Leptin Receptor, KHDRBS1 (KH RNA Binding Domain Containing, Signal Transduction Associated 1), and Adiponectin in Bone Metastasis from Breast Carcinoma: An Immunohistochemical Study.; Authors - Paola Maroni

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