Anticorps Polyclonal de lapin anti-LGR4

LGR4 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 20150-1-AP

Synonymes

G protein coupled receptor 48, GPR48, LGR4

---

Galerie de données de validation

• 

  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 antibody) at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 antibody) at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 20150-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20150-1-AP (LGR4 Antibody) at dilution of 1:200 (under 40x lens).

---

Applications testées

• Résultats positifs en IHC: tissu d'intestin grêle humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

20150-1-AP cible LGR4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LGR4 Protéine recombinante Ag13695
• Nom complet: leucine-rich repeat-containing G protein-coupled receptor 4
• Masse moléculaire calculée: 104 kDa
• Numéro d’acquisition GenBank: BC033039
• Symbole du gène: LGR4
• Identification du gène (NCBI): 55366
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LGR4, also known as GPR48, is a membrane of the leucine-rich GPCR family. LGR4 is composed of a large NH2-terminal extracellular domain containing 18 LRRs, the seven membrane-spanning segments, and a COOH-terminal intracellular domain. LGR4 is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. It has also been shown that LGR4 regulates cyclic AMP/protein kinase A (PKA) signalling. (PMID: 23756652; 17178856; 24212090)

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
human	WB	Mol Ther Oncolytics; Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer.; Authors - Xiaohong Ruan
human	IHC	Cancer Biomark; LGR4 overexpression is associated with clinical parameters and poor prognosis of serous ovarian cancer; Authors - Zhaoyang Zeng
human	IHC	Exp Cell Res; Silencing of RSPO1 mitigates obesity-related renal fibrosis in mice by deactivating Wnt/β-catenin pathway.; Authors - Xuesong Su
mouse	WB	In Vitro Cell Dev Biol Anim; SOX2 suppresses osteoblast differentiation of MC3T3-E1 cells through activating the transcription of LGR4; Authors - Sunyu Chen
pig,mouse	WB	Cell Death Dis; DNA methylation mediated RSPO2 to promote follicular development in mammals.; Authors - Xiaofeng Zhou

{{ptg:RelatedPrimaryAntibodies}}