Anticorps Polyclonal de lapin anti-LGR5

LGR5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 30007-1-AP

Synonymes

FEX, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67

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Galerie de données de validation

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  WB analysis using 30007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30007-1-AP (LGR5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30007-1-AP (LGR5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules BGC-823, cellules HEK-293, cellules SGC-7901, cellules SKOV-3, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

30007-1-AP cible LGR5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LGR5 Protéine recombinante Ag31625
• Nom complet: leucine-rich repeat-containing G protein-coupled receptor 5
• Masse moléculaire calculée: 907 aa, 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC096324
• Symbole du gène: LGR5
• Identification du gène (NCBI): 8549
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67) is a protein that in humans is encoded by the LGR5 gene. It is a member of GPCR class A receptor proteins. R-spondin proteins are the biological ligands of LGR5. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.LGR5 is crucial during embryogenesis as LGR null studies in mice incurred 100% neonatal mortality accompanied by several craniofacial distortions such as ankyloglossia and gastrointestinal dilation (PMID: 9642114, 16575208, 525477).

Publications

Species	Application	Title
mouse	IHC	Burns Trauma; Nocardia rubra cell-wall skeleton mitigates whole abdominal irradiation-induced intestinal injury via regulating macrophage function; Authors - Lingling Wu
mouse	IF	Zool Res; Peptide Cy RL-QN15 accelerates hair regeneration in diabetic mice by binding to the Frizzled-7 receptor; Authors - Yu-Tong Wu
	IF	Int J Biol Macromol; Integrated bulk and single-cell RNA sequencing unveils the temporal and spatial dynamics of epidermal cell adhesion; Authors - Qingbo Zheng
mouse	IF	Acta Histochem; Stimulation of mouse hair regrowth by exosomes derived from human umbilical cord mesenchymal stem cells; Authors - Yang Jiao

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