Anticorps Polyclonal de lapin anti-LGR6

• Phare
• Validé par KD/KO

LGR6 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 17658-1-AP

Synonymes

GPCR, LGR6, VTS20631

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Galerie de données de validation

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  WB analysis of HUVEC using 17658-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17658-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17658-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17658-1-AP (LGR6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17658-1-AP (LGR6 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 17658-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 17658-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LGR6 antibody (17658-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, tissu cérébral humain, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu ovarien humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

17658-1-AP cible LGR6 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LGR6 Protéine recombinante Ag11909
• Nom complet: leucine-rich repeat-containing G protein-coupled receptor 6
• Masse moléculaire calculée: 915 aa, 99 kDa
• Poids moléculaire observé: 104 kDa
• Numéro d’acquisition GenBank: BC047905
• Symbole du gène: LGR6
• Identification du gène (NCBI): 59352
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a seven-transmembrane protein. LGR6 is a high affinity receptor of R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. It potentially functions as a tumor suppressor. (PMID: 20223988; 20417836; 22615920)

Publications

Species	Application	Title
human		J Clin Invest; Maresin 1 activates LGR6 receptor promoting phagocyte immunoresolvent functions.; Authors - Nan Chiang ; KD Validated
human	WB	Mol Ther Oncolytics; Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer.; Authors - Xiaohong Ruan
human	WB	PLoS One; Characterizing the cellular attachment receptor for Langat virus.; Authors - Raquel Rodrigues
mouse	WB	JBMR Plus; Evidence for Lgr6 as a Novel Marker of Osteoblastic Progenitors in Mice.; Authors - Vikram Khedgikar
mouse	ELISA	Proc Natl Acad Sci U S A; The Maresin 1-LGR6 axis decreases respiratory syncytial virus-induced lung inflammation; Authors - Nandini Krishnamoorthy
human	IHC	Aging (Albany NY); Comprehensive analysis of cuproptosis-related immune biomarker signature to enhance prognostic accuracy in gastric cancer; Authors - Jie Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marc (Verified Customer) (02-14-2023)

Used this antibody on immunofluorescence, but the expression of LGR6 in the spinal cord is low so we had to perform an immunofluorescence with tyramide amplification. With tyramide amplification the result is satisfactory.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Spinal Cord