Anticorps Polyclonal de lapin anti-LHCGR

• Phare
• Validé par KD/KO

LHCGR Polyclonal Antibody for FC, IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 19968-1-AP

Synonymes

LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing Hormone Receptor

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Galerie de données de validation

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  WB analysis using 19968-1-AP

  Unboiled mouse testis tissue was subjected to SDS PAGE followed by western blot with 19968-1-AP (LHCGR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19968-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19968-1-AP (LHCGR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19968-1-AP

  IP Result of anti-LHCGR (IP:19968-1-AP, 4ug; Detection:19968-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IF Staining of mouse ovary using 19968-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using LHCGR antibody (19968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 19968-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LHCGR (19968-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris non bouilli, tissu ovarien de souris
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IF: tissu ovarien de souris,
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 3 publications below

Informations sur le produit

19968-1-AP cible LHCGR dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: LHCGR
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: NM_000233
• Symbole du gène: LHCGR
• Identification du gène (NCBI): 3973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lutropin-choriogonadotropic hormone receptor (LHCGR, also known as LHR or LSHR) belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

Publications

Species	Application	Title
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
mouse	WB	Int J Nanomedicine; La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.; Authors - Lu Yuan
human	WB	Am J Pathol; Amyloid Precursor Protein Overexpression in Down Syndrome Trophoblast Reduces Cell Invasiveness and Interferes with Syncytialization.; Authors - Oscar Gee Wan Wong
mouse	WB	PLoS One; The RNA-Binding Protein, ZFP36L2, Influences Ovulation and Oocyte Maturation.; Authors - Christopher B Ball
mouse	WB	Chemosphere; PMID: 28081510; Authors - Xiaoyang Wang
mouse	WB,IHC	Front Endocrinol (Lausanne); Downregulation of LHCGR Attenuates COX-2 Expression and Induces Luteinized Unruptured Follicle Syndrome in Endometriosis.; Authors - Ting Geng

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