Anticorps Polyclonal de lapin anti-LHCGR

• Phare
• Validé par KD/KO

LHCGR Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 26424-1-AP

Synonymes

LSH-R, LHRHR, LHR, LH/CG-R, LGR2

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Galerie de données de validation

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  WB analysis of mouse ovary using 26424-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 26424-1-AP (LHCGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 26424-1-AP (LHCGR antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse ovary using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse ovary using 26424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using LHCGR antibody (26424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu ovarien de souris
• Résultats positifs en IHC: human ovary cancer tissue, tissu de cancer de la thyroïde humain, tissu ovarien de souris, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu ovarien de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

26424-1-AP cible LHCGR dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LHCGR Protéine recombinante Ag19259
• Nom complet: LHCGR
• Masse moléculaire calculée: 699 aa, 79 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC157028
• Symbole du gène: LHCGR
• Identification du gène (NCBI): 3973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LHCGR, also known as LHR or LSHR, belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

Publications

Species	Application	Title
		Biol Reprod; Interferon-tau infusion into the ovine corpus luteum delays luteolysis; Authors - Aydin Guzeloglu
mouse	WB,IF	Biochem Biophys Res Commun; Effects of hCG on DA neuronal death of Parkinson's disease; Authors - Shi-Min Wang ; KD Validated
mouse	IF	Biol Res; Mouse testicular macrophages can independently produce testosterone and are regulated by Cebpb; Authors - Nengliang Duan

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