Anticorps Polyclonal de lapin anti-LHPP

• Phare
• Validé par KD/KO

LHPP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 15759-1-AP

Synonymes

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, hLHPP, EC:3.1.3.-

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Galerie de données de validation

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  WB analysis using 15759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15759-1-AP

  WB result of LHPP antibody (15759-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LHPP transfected A549 cells.

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  WB analysis of mouse liver using 15759-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15759-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15759-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15759-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15759-1-AP (LHPP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15759-1-AP (LHPP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15759-1-AP (LHPP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15759-1-AP (LHPP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15759-1-AP

  Immunofluorescent analysis of Hela cells, using LHPP antibody 15759-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules A431, cellules A549, cellules HeLa, cellules HepG2, tissu cérébral humain, tissu hépatique de rat, tissu hépatique humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 14 publications below
• IHC: See 9 publications below
• IF: See 2 publications below

Informations sur le produit

15759-1-AP cible LHPP dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LHPP Protéine recombinante Ag8375
• Nom complet: phospholysine phosphohistidine inorganic pyrophosphate phosphatase
• Masse moléculaire calculée: 23 kDa, 29 kDa
• Poids moléculaire observé: 23-32 kDa
• Numéro d’acquisition GenBank: BC007324
• Symbole du gène: LHPP
• Identification du gène (NCBI): 64077
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Mol Cancer; YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.; Authors - Jiangfeng Li
human	WB,IHC	Cell Death Dis; Phosphatase LHPP confers prostate cancer ferroptosis activation by modulating the AKT-SKP2-ACSL4 pathway; Authors - Guoqing Xie
human	WB	Biochim Biophys Acta Mol Basis Dis; Correlated with better prognosis, CSTA inhibits metastasis of nasopharyngeal carcinoma cells via suppressing AKT signaling through promoting METTL3 degradation; Authors - Zhi-Jie Liu
human	WB	Aging (Albany NY); The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis.; Authors - Xuhui Yang ; KD Validated
human	WB	Cell Cycle; LHPP inhibits hepatocellular carcinoma cell growth and metastasis.; Authors - Lijuan Liao
human	WB,IHC,IF	Oncol Rep; Tumor suppressor LHPP regulates the proliferation of colorectal cancer cells via the PI3K/AKT pathway.; Authors - Bin Hou

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