Anticorps Polyclonal de lapin anti-LIF

LIF Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 26757-1-AP

Synonymes

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

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Galerie de données de validation

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  WB analysis of Jurkat using 26757-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26757-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 26757-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26757-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 26757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 26757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse embryo using 26757-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using LIF antibody (26757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu embryonnaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu embryonnaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

26757-1-AP cible LIF dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIF Protéine recombinante Ag25076
• Nom complet: leukemia inhibitory factor (cholinergic differentiation factor)
• Masse moléculaire calculée: 202 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC093733
• Symbole du gène: LIF
• Identification du gène (NCBI): 3976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

Publications

Species	Application	Title
human	IF	Cell Stem Cell; LIF signaling regulates outer radial glial to interneuron fate during human cortical development; Authors - Madeline G Andrews
mouse	WB,IHC	Toxicology; Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice; Authors - Yong-Jiang Zhou
human	WB	Hum Reprod; The depot GnRH agonist protocol improves the live birth rate per fresh embryo transfer cycle, but not the cumulative live birth rate in normal responders: a randomized controlled trial and molecular mechanism study.; Authors - Bei Xu
human	IHC	Int J Cancer; Leukemia inhibitory factor is a novel biomarker to predict lymph node and distant metastasis in pancreatic cancer.; Authors - Wenna Jiang
human	WB	Reprod Biol Endocrinol; Expression and significance of miR-30d-5p and SOCS1 in patients with recurrent implantation failure during implantation window; Authors - Yuhao Zhao
mouse	IHC	Reproduction; Consistency and synchronization of AMPK-glycogen in endometrial epithelial cells are critical to the embryo implantation.; Authors - Li Nie